17年4月21日，加拿大多伦多大学副校长Bernie Kraatz教授讲座
发布日期：2017年04月21日 阅读： 次
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| 加拿大多伦多大学副校长Bernie Kraatz教授讲座 题目：Electrochemical Studies of Nucleic Acids on Surfaces: Towards the Identification of Species 时间：2017年 4 月21日 16点30 到 17点 30 地点： 环境馆一楼会议室 主持： 曾光明 院长教授、汤琳 副院长教授 欢迎有兴趣的老师、研究生、本科生参加。 ------------------------ 加拿大多伦多大学副校长Bernie Kraatz教授讲座内容： Electrochemical Studies of Nucleic Acids on Surfaces: Towards the Identification of Species. Bernie Kraatz Department of Physical and Environmental Sciences, University of Toronto Scarborough; Department of Chemistry, University of Toronto, Toronto, Canada; bernie.kraatz@utoronto.ca The detection of biological analytes or of biochemical processes by electrochemical methods requires in many cases the presence of a redox-active probe as part of the detection system. The focus of this presentation is on the use of electrochemical methods to probe nucleic acids without the need to label DNA targets. My research group has developed an impedance-based assay that allows the detection of single nucleotide mismatches. This assay does not require any labeling of the target strand and relies on monitoring changes in charge transfer resistance of an external anionic redox probe as a function of basepair mismatch. The effect is caused by differences in the ability of the redox probe to diffuse into a DNA film. Furthermore, the use of scanning electrochemical microscopy (SECM) for the study of nucleobase mismatches will be discussed. SECM is ideal to study these effects since the currents are limited by the re-generation of the redox mediator at the modified electrode surface. Here we report that we can unequivocally identify the presence and position of single nucleotide basepair mismatches using ferricyanide as a solution-based redox mediator. Our results show that the presence of metal ions enhances the detection by SECM significantly. Environmental problems are explicit in many forms in nature and are considered to be the most pressing issue concern in the future of human survival. They can often be detected and transported as pollutants in water, soil and air. Solving these problems requires a systematic method contributed by environmental scientists with different expertise. The techniques require to solve these environmental issues. This multi-disciplinary approach to clean our environment requires advanced professional trainings that are offered through the Master of Environmental Science at the University of Toronto Scarborough. 加拿大多伦多大学副校长Bernie Kraatz教授个人简介： Bernie (H.-B.) Kraatz (PhD 1993, U. Calgary). Professor of Chemistry and Vice-Principal Research (Scopus Author ID: 35464724800). He has received several awards including the PetroCanada Young Innovator Award (2000), the Canadian Research Chair in Biomaterials (2001), and in 2006 the Award in Pure or Applied Inorganic Chemistry from the Canadian Society for Chemistry. In addition, he received the Florence Bucke Science Prize (2009) and became Faculty Scholar and a Distinguished Research Professor (2010). He received the Principals Research Award (2015) and most recently is the recipient of the Rio Tinto Alcan Award from the Canadian Society for Chemistry. His research is focused on the design of biosensors, surface-supported functional bioconjugates, and bio(nano)materials. He has pioneered work on redox-active peptides, explored their self-assembly properties and exploited them for monitoring protein binding and protein or even whole cell biosensing by electrochemical methods. He works the fabrication of chip-based electrochemical sensors for mismatch detection in DNA, nucleobase sequencing, protein detection, and monitoring enzymatic activities and is working on multidimensional and multifunctional sensor arrays. He published more than 250 research papers and currently has an h-factor of 46. For more information please see http://www.utsc.utoronto.ca/~bkraatz/ or https://orcid.org/0000-0002-7149-0110  |
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